產(chǎn)品效果
形態(tài)完整
粒徑均一
鑒定合格
操作方法
轉(zhuǎn)移樣本至新管,加入與血清/血漿等體積的試劑;
渦旋混勻,此步后溶液將變混濁。
放入4℃冰箱靜置過夜;
4℃,3220g離心60min,棄上清,外泌體沉淀存留在離心管底部;
用適量PBS重懸外泌體沉淀,存放于4℃(不超過1周)或-20℃/-80℃(長時間保存)
參考文獻(xiàn)
Wang et al. Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients. Signal Transduction and Targeted Therapy. 2021
Jin et al. A novel class of tsRNA signatures as biomarkers for diagnosis and prognosis of pancreatic cancer. Molecular Cancer. 2021
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